IgY An Alternative antibody. This text book gives a clear illustration and description of the principles and concepts of immunology in a highly accessible and comprehensive manner. This book is designed to cater the basic needs of the scientists in the field of Biomedical science, Biotechnology, Immunology, Biochemistry and Microbiology. This book also elaborates and highlights the various techniques employed for in vivo and in vitro analysis of biomolecules. Each chapter starts with introductory details of the techniques followed by laboratory methods. The book is compartmented into five following chapters, 1. Introduction 2. Venom composition and enzyme activities 3. Symptoms, signs and treatment of cobra bite 4. Extraction of egg yolk IgY 5.In vivo and In vitro assays.

IgY An Alternative antibody. This text book gives a clear illustration and description of the principles and concepts of immunology in a highly accessible and comprehensive manner. This book is designed to cater the basic needs of the scientists in the field of Biomedical science, Biotechnology, Immunology, Biochemistry and Microbiology. This book also elaborates and highlights the various techniques employed for in vivo and in vitro analysis of biomolecules. Each chapter starts with introductory details of the techniques followed by laboratory methods. The book is compartmented into five following chapters, 1. Introduction 2. Venom composition and enzyme activities 3. Symptoms, signs and treatment of cobra bite 4. Extraction of egg yolk IgY 5.In vivo and In vitro assays.

In this book, Enterotoxigenic Escherichia coli (ETEC) is described as one of the pathogenic bacteria that is responsible for diarrhoea in human and farm animals. Several fimbrial adhesins of ETEC strains (K88, K99,987P and F41) were used as the primary character of ETEC diarrhoea. Regular dosages of combined K88-K99-987P and K88-K99-F41 through 4 commercial adjuvants: Freund's, White oil, Al(OH)3, and Phosphate Buffer Saline (PBS) were immunised in chickens for IgY antibody production. IgY antibody extraction and purification methods from the eggs were devised. After HiTrap affinity column purification, SDS-PAGE confirmed that the extracted IgY was electrophoretic clean with a molecular weight of 188 kDa. ELISA test indicated that the induced IgYs were 5 titers higher as compared to the non-immunized chickens. The richness of lecithin and cholesterol in egg yolk was found to get the unpurified IgY stable throughout the stomach pH (3-4). Complexed IgY-egg powder (given in-vivo) as a novel food powder could transit the piglet's stomach and had a curative effect against ETEC-inducing diarrhoea.

In this book, Enterotoxigenic Escherichia coli (ETEC) is described as one of the pathogenic bacteria that is responsible for diarrhoea in human and farm animals. Several fimbrial adhesins of ETEC strains (K88, K99,987P and F41) were used as the primary character of ETEC diarrhoea. Regular dosages of combined K88-K99-987P and K88-K99-F41 through 4 commercial adjuvants: Freund's, White oil, Al(OH)3, and Phosphate Buffer Saline (PBS) were immunised in chickens for IgY antibody production. IgY antibody extraction and purification methods from the eggs were devised. After HiTrap affinity column purification, SDS-PAGE confirmed that the extracted IgY was electrophoretic clean with a molecular weight of 188 kDa. ELISA test indicated that the induced IgYs were 5 titers higher as compared to the non-immunized chickens. The richness of lecithin and cholesterol in egg yolk was found to get the unpurified IgY stable throughout the stomach pH (3-4). Complexed IgY-egg powder (given in-vivo) as a novel food powder could transit the piglet's stomach and had a curative effect against ETEC-inducing diarrhoea.

The protocol developed for IgY purificationindicated approximately 74% recovery rate and 80%purity, while that of ovotransferrin appeared to bearound 93% recovery rate and 80% purity. Theseprotocols seem to be economical and practical for alarge-scale purification of IgY and ovotransferrinfrom chicken egg because ethanol and resin used inthe protocols can be regenerated as well as they aresimple and rapid methods. The combination of 100 mM-NaHCO3 to ovotransferrin was found to bebacteriostatic against E. coli O157:H7 and L.monocytogenes in BHI broth. Also ovotransferrinsolution added with 0.5% citric acid or Zn-boundovotransferrin inhibited the growth E. coli O157:H7and L. monocytogenes in BHI broth respectively.Also, EDTA and lysozyme promoted antibacterialactivity of ovotransferrin solution combined with100 mM-NaHCO3 against E. coli O157:H7 andL.monocytogenes respectively. Based on theseresults, this study claims that ovotransferrincombined citric acid, NaHCO3, and EDTA or lysozymeseemed to be useful antibacterial agents forcontrolling E. coli O157:H7 and L. monocytogenes.

The protocol developed for IgY purificationindicated approximately 74% recovery rate and 80%purity, while that of ovotransferrin appeared to bearound 93% recovery rate and 80% purity. Theseprotocols seem to be economical and practical for alarge-scale purification of IgY and ovotransferrinfrom chicken egg because ethanol and resin used inthe protocols can be regenerated as well as they aresimple and rapid methods. The combination of 100 mM-NaHCO3 to ovotransferrin was found to bebacteriostatic against E. coli O157:H7 and L.monocytogenes in BHI broth. Also ovotransferrinsolution added with 0.5% citric acid or Zn-boundovotransferrin inhibited the growth E. coli O157:H7and L. monocytogenes in BHI broth respectively.Also, EDTA and lysozyme promoted antibacterialactivity of ovotransferrin solution combined with100 mM-NaHCO3 against E. coli O157:H7 andL.monocytogenes respectively. Based on theseresults, this study claims that ovotransferrincombined citric acid, NaHCO3, and EDTA or lysozymeseemed to be useful antibacterial agents forcontrolling E. coli O157:H7 and L. monocytogenes.

Erscheinungsdatum: 05/2012, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: IgY - An Alternative Antibody, Titelzusatz: Extraction and validation, Autor: Prabhu, R. Rajendra, Verlag: LAP Lambert Academic Publishing, Sprache: Englisch, Rubrik: Biochemie // Biophysik, Seiten: 72, Informationen: Paperback, Gewicht: 124 gr, Verkäufer: averdo

Manfrotto MB NX-P-IGY-2. Etui-Typ: Holster, Markenkompatibilität: Universal, Kompatibilität: Canon EOS M10, Canon EOS M100, Canon EOS M3, Fujifilm X-A2, Fujifilm X-A3, Fujifilm X-E2S,..., Produktfarbe: Grau

The 'Year' That Changed How We View the North This book is about a new theoretical approach that transformed the field of Arctic social studies and about a program called International Polar Year 2007-2008 (IPY) that altered the position of social research within the broader polar science. The concept for IPY was developed in 2003-2005, its vision was for researchers from many nations to work together to gain cro- disciplinary insight into planetary processes, to explore and increase our understanding of the polar regions, the Arctic and Antarctica, and of their roles in the global system. IPY 2007-2008, the fourth program of its kind, followed in the footsteps of its predecessors, the first IPY in 1882-1883, the second IPY in 1932-1933, and the third IPY (later renamed to 'International Geophysical Year' or IGY) in 1957-1958. All earlier IPY/IGY have been primarily geophysical initiatives, with their focus on meteorology, atmospheric and geomagnetic observations, and with additional emphasis on glaciology and sea ice circulation. As such, they excluded socio-economic disciplines and polar indigenous people, often deliberately, except for limited ethnographic and natural history collection work conducted by some expeditions of the first IPY. That once dominant vision biased heavily towards geophysics, oceanography, and ice-sheets, left little if any place for people, that is, the social sciences and the humanities, in what has been commonly viewed as the 'hard-core' polar research.